Swine TNF-Alpha ELISA Assay

Product Overview:

The swine TNF-alpha ELISA assay (enzyme-linked immunoassay kit) is intended for the quantitative determination of porcine tumor necrosis factor alpha (TNA-alpha) concentrations in cell culture supernates, serum, and plasma.

Product Description:

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin, is a member of the TNF ligand superfamily and has been designated TNFSF1A. It binds to the same cell surface receptors, and shares some biological functions with TNF-b/TNFSF1B. TNF-alpha inhibits the growth of certain tumors. It also plays a critical role in normal host resistance to infection, serving as an immunomodulator and as a mediator of inflammatory responses. Over-production of TNF has been implicated in a number of pathological conditions, including cachexia, septic shock, and autoimmune disorders. TNF-alpha is produced primarily by activated macrophages. Various other porcine cell types, including NK cells, keratinocytes, vascular smooth muscle cells, and granulosa lutein cells are also known to produce TNF-alpha.

Sensitivity:

15 pg/ml

Dynamic Range:

31.25 – 1000 pg/ml

Incubation Time:

3 hours

Sample Type:

Serum, plasma, cell culture

Sample Size:

100 µl